Prof. Hongyuan CHEN

hychen@nju.edu.cn

 

In this project we started with the study of functionally modified interface of electrodes, used some sig-nificant biomolecules as model substances including nucleic acids, proteins (enzymes), neurotransmit-ters, etc., introduced nanotechnology and biochemical technology, did systematic researches on many different matrixes and types of electrode-systems in order to prepare bionic catalytic interface and bio-sensors, and established some new kinds of electroanalytical methods.

 

In the study of microelectrode theory and applications, we introduced some new concepts, discovered some new effects, figured out a series of expressions of steady-state current in complicated systems, and deduced the current expression of the microband array electrode and its measurement method. We established more than ten new kinds of micro-biosensors to sensitively determine biomolecules based on micro-disk, micro-column, micro-band and micro-band array electrodes. In the study of the basis and applications of bioelectrochemistry, we fabricated biosensors with different kinds of films, includ-ing organic polymer films, inorganic coordination compound films and self-assembly monolayer film modified electrodes and established high sensitive and selective analytical methods to determine nucleic acids, proteins (enzymes), coenzymes and small biomolecules.

 

We took the lead in modifying nanoparticles on the surface of electrode as an electron wire. It could promote the interface reaction of enzymes and proteins, and make the fixed biomolecules keep their bio-activity for a long time via its bridge-joint effect. We invented several methods to fabricate nano-bionic interfaces, and constructed a set of biosensors with fast response, high sensitivity and long lifetime. In addition, we initiated the assembly of nanoparticles on the insulation gate surface of the field-effect tran-sistors and successfully made the first field-effect transistor biosensor modified by nanoparticles. More over, we discovered the electron-wire effect amplification property and new reaction characteristics of nanoparticles, which were used to fabricate biosensors with improved performances.

 

In the part of new electrochemical detection method coupled with flow systems, some sensitive ana-lytical systems to detect trace enzymes, nucleic acids, purines, amino acids, oligosaccharides, neuro-transmitters, and organic acids etc. by the capillary electrophoresis with electrochemical detection were established. A novel electrochemical detection approach was used that can transform the interfering ef-fect of separation electric field into a universal analytical method. Such a method has been successfully applied to the detection of not only electroactive but also nonelectroactive analytes, as well as electroos-motic flow rates in microfluidic chip electrophoresis systems.

 

This project published 140 papers on SCI journals, which were cited 2250 times by SCI papers. Among the citations, 1887 times are from others. The representative 10 papers have been cited by others for 330 times. The highest cited time of one paper is 95. The authors citing our results come from over 20 coun-tries, including USA, England, Germany ,France and Japan etc. The main achievers have been invited to write 5 reviews to report some results of this project. It cultivated 51 graduated students (Ph. D: 39, Master: 12).